电离辐射通过转化生长因子-β-介导的上皮-间质转换成来促进癌细胞的侵袭迁移

Int J Radiat Oncol Biol Phys 2011 Dec;81 (5): 1530-7. [IF:4.503]Ionizing radiation promotes migration and invasion of cancer cells through transforming growth factor-Beta-mediated epithelial-mesenchymal transition.Zhou YC , Liu JY , Li J , Zhang J , Xu YQ , Zhang HW , Qiu LB , Ding GR , Su XM , Mei-Shi , Guo GZ .Department of Radiation Oncology, Xijing Hospital Fourth Military Medical University, Xi'an, China; Department of Radiation Medicine, College of Preventive Medicine, Xijing Hospital Fourth Military Medical University, Xi'an, China.第四军医大学西京医院放射科

AbstractTo examine whether ionizing radiation enhances the migratory and invasive abilities of cancer cells through transforming growth factor (TGF-β)-mediated epithelial-mesenchymal transition (EMT). Six cancer cell lines originating from different human organs were irradiated by (60)Co γ-ray at a total dose of 2 Gy, and the changes associated with EMT, including morphology, EMT markers, migration and invasion, were observed by microscope, Western blot, immunofluorescence, scratch assay, and transwell chamber assay, respectively. Then the protein levels of TGF-β in these cancer cells were detected by enzyme-linked immunosorbent assay, and the role of TGF-β signaling pathway in the effect of ionizing radiation on EMT was investigate by using the specific inhibitor SB431542. After irradiation with γ-ray at a total dose of 2 Gy, cancer cells presented the mesenchymal phenotype, and compared with the sham-irradiation group the expression of epithelial markers was decreased and of mesenchymal markers was increased, the migratory and invasive capabilities were strengthened, and the protein levels of TGF-β were enhanced. Furthermore, events associated with EMT induced by IR in A549 could be reversed through inhibition of TGF-β signaling. These results suggest that EMT mediated by TGF-β plays a critical role in IR-induced enhancing of migratory and invasive capabilities in cancer cells.

内容可 ：探讨辐射源是不是可通过裂解生长因子-β（TGF-β）-介导的粘液-间质匹配 (EMT)来促进肿瘤肝细胞的首当其冲迁移。用于分之一2Gy(60)Coγ线照射到源自人类心脏的6种肿瘤肝细胞，记录与EMT相关的变化，这包括分别利用显微镜应用，蛋白质区别于方法，免疫荧光应用，划痕试验性和Transwell小室试验性来掩蔽并检测肝细胞分组织形态，EMT标示，首当其冲迁移能力等。运用于酶同德免疫吸附法检测这些肿瘤肝细胞里面TGF-β蛋白准确度，利用特别减缓剂SB431542来评核TGF-β接收机自营在辐射源EMT里面的发挥作用。经过分之一为2Gy照射到的肿瘤肝细胞里面假定间叶肝细胞的表达，与所谓照射到分组相比其粘液标示减少，间叶肝细胞标示增加，同时其首当其冲移到能力加强，TGF-β蛋白准确度也进一步提高。进一步发现由A549辐射源诱导的EMT可通过对TGF-β接收机减缓发生逆转。这些最近TGF-β介导的EMT在辐射源诱导加强肿瘤肝细胞首当其冲移到能力里面起着关键发挥作用。

主编： lizexiu